Journal: Antimicrobial Agents and Chemotherapy
Article Title: Incorporation of macrophage immune stresses into an intracellular assay of drug tolerance in Mycobacterium tuberculosis
doi: 10.1128/aac.00795-25
Figure Lengend Snippet: Mtb drug susceptibility to INH, RIF, EMB, and PZA in LPS/IFNγ-activated BMDM h s. Resting and activated macrophages were infected with the Mtb Erdman-Lux strain at an MOI of 0.4 for 4 days before addition of drugs. Drugs were added on day 4 (indicated by arrow). Macrophage media were replaced with 7H9 broth on day 8 (4 days after addition of the drugs). Bacterial growth was monitored by measuring luciferase relative light units (RLUs). Growth kinetics of Mtb in macrophages treated with the indicated concentrations of INH ( A ), RIF ( B ), EMB ( C ), and PZA ( D ). n = 3 biological replicates. *** P < 0.001, **** P < 0.0001, two-way analysis of variance (ANOVA) alongside Dunnett’s multiple comparison test. Data are presented as mean values ± standard deviation (SD).
Article Snippet: The Mtb Erdman strain (ATCC 35801) was used in CFU experiments.
Techniques: Infection, Luciferase, Comparison, Standard Deviation